Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Sep 11;10(17):5223-38.
doi: 10.1093/nar/10.17.5223.

A yeast transcription system for the 5S rRNA gene

H van Keulen, D Y Thomas

A yeast transcription system for the 5S rRNA gene

H van Keulen et al. Nucleic Acids Res. .

Abstract

A cell-free extract of yeast nuclei that can specifically transcribe cloned yeast 5S rRNA genes has been developed. Optima for transcription of 5S rDNA were determined and conditions of extract preparation leading to reproducible activities and specificities established. The major in vitro product has the same size and oligonucleotide composition as in vivo 5S rRNA. The in vitro transcription extract does not transcribe yeast tRNA genes. The extract does increase the transcription of tRNA genes packaged in chromatin.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 - PubMed
    1. Methods Cell Biol. 1975;12:45-64 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1024-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1029-33 - PubMed
    1. Anal Biochem. 1976 May 7;72:433-46 - PubMed