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. 1982 Sep 25;10(18):5605-20.
doi: 10.1093/nar/10.18.5605.

Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides

T P Patel, T A Millican, C C Bose, R C Titmas, G A Mock, M A Eaton
Free PMC article

Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotides

T P Patel et al. Nucleic Acids Res. .
Free PMC article

Abstract

A solution of benzenesulphonic acid (3%, w/v) in a dimethylformamide and dichloromethane mixture (9:1, v/v) is shown to be a very effective reagent for the detritylation of deoxyoligonucleotides attached to a solid support. The levels of depurination with this reagent were lower than those observed with other reagents such as trichloroacetic acid. Coupling reactions are described using above ambient temperatures with no detectable increase in side products. Both procedures have been successfully incorporated into an automated system, which can compete with the rate of synthesis by the phosphite approach.

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