doi: 10.1016/0014-5793(82)81116-2.
The effect of Ca2+ on the stability of chicken erythrocyte histone octamers
- PMID: 7152043
- DOI: 10.1016/0014-5793(82)81116-2
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The effect of Ca2+ on the stability of chicken erythrocyte histone octamers
FEBS Lett.
.
Free article
Abstract
Histones can be extracted from chicken erythrocyte chromatin with 2 M CaCl2 10 mM Tris (pH 7.4). The core histones so extracted exist as H3-H4 tetramers and H2A-H2B dimers since calcium at greater than 0.5 M result in dissociation of the histone octamer. Reconstitution of octamers occurs on removal of the Ca2+ by dialysis. Although less than 0.5 M calcium do not result in octamer dissociation, perturbations in the structure can be detected by CD and tyrosine fluorescence spectroscopy.
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