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. 1982 Dec;7(1):15-23.
doi: 10.1016/0165-022x(82)90032-x.

A method for hydrolyzing and determining the amino acid compositions of picomole quantities of proteins in less than 3 hours

G L Lookhart, B L Jones, D B Cooper, S B Hall

A method for hydrolyzing and determining the amino acid compositions of picomole quantities of proteins in less than 3 hours

G L Lookhart et al. J Biochem Biophys Methods. 1982 Dec.

Abstract

A method is described for quantitatively hydrolyzing proteins in 45 min and for analyzing the hydrolysates by high-performance liquid chromatography in an additional 52 min. The alpha-amino acids were detected by the fluorescence of their omicron-phthaldialdehyde derivatives. Ten picomoles of each of the commonly occurring alpha-amino acids could be reliably determined. The method described yielded OPA-ethanethiolamino acid derivatives that were stable for 1 1/2 h and the HPLC method produced a better separation than previously published methods.

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