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. 1978 Sep;27(5):1061-4.
doi: 10.4269/ajtmh.1978.27.1061.

Growth of Babesia bovis in bovine erythrocyte cultures

E E Erp, S M Gravely, R D Smith, M Ristic, B M Osorno, C A Carson

Growth of Babesia bovis in bovine erythrocyte cultures

E E Erp et al. Am J Trop Med Hyg. 1978 Sep.

Abstract

Babesia bovis was cultured in a suspension of bovine erythrocytes incubated at 37 degrees C in Medium 199 with 50% bovine serum. The cells in culture were kept in suspension by slow stirring in spinner flasks and the medium was replaced at 24-hour intervals. Persistent multiplication of the parasite in a short series of subcultures suggests the feasibility of this approach for continuous culture.

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