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Comparative Study
. 1982:(11):173-6.

Single crystals of 5S rRNA from Thermus thermophilus HB8 and preliminary X-ray diffraction data

K Morikawa, M Kawakami, S Takemura
  • PMID: 7183958
Comparative Study

Single crystals of 5S rRNA from Thermus thermophilus HB8 and preliminary X-ray diffraction data

K Morikawa et al. Nucleic Acids Symp Ser. 1982.

Abstract

5S rRNA from extremely thermophilic bacteria, Thermus thermophilus HB8, was crystallized. Using a vapour diffusion method, single crystals were grown with sufficient sizes for x-ray diffraction study. The crystals belong to space group P3(1)21 (or enatiomorph P3(2)21) with a = 99 A, b = 99 A and c = 360 A. A unit cell contains probably 36 molecules corresponding to 6 molecules/asymmetric unit. A smaller pseudo-lattice seems to be constructed with the same lengths for a- and b-axis, and 120 A for c-axis. Reflections were observed up to 25 A on x-ray photographs.

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