Effect of cholesterol on the molecular motion in the hydrocarbon region of lecithin bilayers studied by nanosecond fluorescence techniques

Abstract

Effects of cholesterol on the dynamic structure of the hydrocarbon region of dipalmitoyllecithin vesicles were examined. Decays of the emission anisotropy and the fluorescence intensity of 1,6-diphenyl-1,3,5-hexatriene embedded in lecithin-cholesterol vesicles were measured over a temperature range of 10--60 degrees C. The emission anisotropy decreased rapidly with time and then leveled off. The rotational motion of the probe was analyzed by a model of wobbling diffusion confined in a cone. Cholesterol (10--50 mol%) decreased the cone angle in the liquid-crystalline phase and increased it in the gel phase. In the presence of 33 mol% cholesterol, the wobbling diffusion constant increased in the gel phase and changed little in the liquid-crystalline phase. The viscosity in the cone decreased in the gel phase and remained almost unchanged in the liquid-crystalline phase in the presence of 33 mol% cholesterol. The total fluorescence intensity followed a singel exponential decay independently of the cholesterol content 0--50 mol%.