Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei

Abstract

Ovalbumin is synthesized by Xenopus oocytes injected with a plasmid containing either the chicken chromosomal ovalbumin gene or a plasmid from which the 5' region of the chromosomal gene has been deleted. However, oocytes injected with a plasmid containing full-length ovalbumin cDNA do not synthesize ovalbumin, despite the fact that at least as much stable, ovalbumin-specific RNA is transcribed from the cDNA as from the chromosomal gene.