[Chromosome aberrations induced by cyclophosphamide in the meiotic cells of male mice]

Abstract

Cyclophosphamide was given intraperitoneally in doses of 40, 80 and 160 mg/kg to C57BL/6 male mice. Cytogenetic effect in spermatogonia of A and B types and in pachytene spermatocytes was studied by the frequencies of chromosomes recorded in cells at the stage of diakinesis MI meiosis. In some cells cyclophosphamide induced structural chromosomal aberrations, including translocations and the disturbances of normal bivalent formation. The dose of 80 mg/kg induced translocations in spermatocytes with frequency 0.6% and appearance of 3% of translocation carriers in F1 generation. Three males were heterozygotes by the translocation of the Robertsonian type.