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. 1980 Oct;18(4):506-10.
doi: 10.1128/AAC.18.4.506.

Enzymatic method for rapid and sensitive determination of beta-lactam antibiotics

J M Frère, D Klein, J M Ghuysen

Enzymatic method for rapid and sensitive determination of beta-lactam antibiotics

J M Frère et al. Antimicrob Agents Chemother. 1980 Oct.

Abstract

A rapid and sensitive procedure for the estimation of beta-lactam antibiotics is described which makes use of the ability of these antibiotics to inactivate the R39 DD-carboxypeptidase. Depending on the values of the kinetic parameters which govern the reaction, the antibiotics fall into two groups. The lower limit for the quantitative estimation of the antibiotics of groups I and II is about 5 and 50 pmol/ml, respectively. The procedure has been adopted to biological fluids such as human sera and cows' milk.

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References

    1. Appl Microbiol. 1966 Mar;14(2):170-7 - PubMed
    1. Antimicrob Agents Chemother. 1973 Apr;3(4):463-8 - PubMed
    1. Biochem J. 1974 Oct;143(1):233-40 - PubMed
    1. Biochem J. 1976 Jun 1;155(3):623-9 - PubMed
    1. Methods Enzymol. 1976;45:610-36 - PubMed

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