Micro method for performing titration and neutralization test of hog cholera virus using established porcine kidney cell strain

Abstract

Hog cholera (HC) virus and antibody against it were estimated by the END method with microplates and CPK porcine kidney cell strain. To establish the technique of this method, studies were made on such basic conditions of the method as the type of strain of Newcastle disease virus (NDV), the time of challenge with this virus, and the concentration of serum in culture fluid. There was little difference in the infective titer of HC virus estimated between the END method performed by the established technique and the same method by the conventional technique with test tubes and swine testicle (ST) cells. Besides, there was a high correlation between the neutralizing antibody titer measured by the one technique and that measured by the other. The coefficient of correlation was r = +0.948 in this case. From the experimental results mentioned above it was concluded that the END method by the micro-technique with CPK cells was simpler than and as reliable as the same method conducted by any conventional technique, and that it was a practicable one capable of testing many samples.