Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982;40(1):45-50.

[Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects (author's transl)]

[Article in French]
  • PMID: 7200750

[Radioimmunoassay for prostatic acid phosphatase in human serum. Methodologic aspects (author's transl)]

[Article in French]
N Pradalier et al. Ann Biol Clin (Paris). 1982.

Abstract

We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A specific antibody is raised in rabbits and purified by immunoadsorption with a female serum. The described technique offers both radioisotopic sensibility and immunologic specificity. Physiological values determined in the serum of 125 healthy males are below 2 ng/ml. No significative differences are observed with age. The proposed technique also shows significant differences between values evaluated for benign prostatic hyperplasia and prostatic adenocarcinoma.

PubMed Disclaimer

Similar articles

Publication types

Substances

LinkOut - more resources