Pregnancy stimulates DNA synthesis in R3230AC mammary adenocarcinoma

Abstract

The transplantable R3230AC mammary adenocarcinoma was grown in virgin, pregnant and lactating rats and in vivo rates of [3H]-thymidine incorporation into DNA and acid-soluble dTTP were compared between tumor and host mammary glands. The tumor differed from the host gland in that the rates of uptake and phosphorylation of the injected thymidine remained unchanged throughout the lactation cycle, but the dTTP pool increased greatly during pregnancy and declined during lactation. In both tumor and host gland, DNA labeling rates were higher during pregnancy than during lactation. Tumor DNA synthesis rates, in terms of incorporated dTMP, increased markedly during pregnancy and returned to pre-pregnant rates following parturition and during lactation. This pattern was similar to host mammary glands, but the change was of a greater magnitude. The data illustrate the usefulness of growing a transplantable mammary tumor in rats of varying physiological states. This way, similarities and differences between a mammary tumor and the host mammary gland regarding their responses to the hormonal milieu of pregnancy and lactation can be assessed.