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. 1978 Nov 15;34(11):1515-7.
doi: 10.1007/BF01932385.

Isolation of a growth-stimulating agent from human skin fibroblast cultures

A A Hakim

Isolation of a growth-stimulating agent from human skin fibroblast cultures

A A Hakim. Experientia. .

Abstract

Cell-free supernatants were harvested from cultures of human skin fibroblasts, were applied on to DEAE-cellulose columns, and the first fraction eluted with phosphate-buffered saline contained the growth-stimulating agent. The eluted fraction was then passed through a series of amicon membranes. After passing through PM-10, the filtrate stimulated growth of bovine vascular endothelial, canine myocardial, and human mammary carcinoma cells.

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References

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