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. 1978 Sep 15;34(9):1149-51.
doi: 10.1007/BF01922924.

Demonstration of the presence of M-creatine kinase in mammalian myogenic cell lines

J C Perriard, H M Eppenberger

Demonstration of the presence of M-creatine kinase in mammalian myogenic cell lines

J C Perriard et al. Experientia. .

Abstract

Unequivocal identification of M-CK in cell extracts from fused cells of myogenic cell lines is difficult due to almost identical behaviour of the M-CK and a contaminating enzyme activity in electrophoresis. If CK dimers present in cell extracts were subjected to dissociation and reassociation in the presence of exogenous B-CK subunits, the formation of easily identifiable MB-CK was demonstrated, indicating the presence of M-CK in the myogenic rat cell lines.

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