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. 1980 Sep 1;189(3):541-6.
doi: 10.1042/bj1890541.

The electrophoresis of transferrins in urea/polyacrylamide gels

R W Evans, J Williams

The electrophoresis of transferrins in urea/polyacrylamide gels

R W Evans et al. Biochem J. .

Abstract

The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations.

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