Comparison of nucleotide sequences of the early E1a regions for subgroups A, B and C of human adenoviruses

Abstract

The early E1a regions (map position (0--4.5%) of highly oncogenic (subgroup A) adenovirus serotype 12 (Ad12), weakly oncogenic (subgroup B) adenovirus serotype 7 (Ad7) and non-oncogenic (subgroup C) adenovirus serotype 5 (Ad5) can convert cells in vitro into an immortal cell line. A comparison of the nucleotide sequences for the E1a regions of Ad12 (Sugisaki et al., 1980), Ad7 (Dijkema et al., 1980), and Ad5 (Van Ormondt et al., 1978) DNA is presented. The overall homology is about 50%, but certain segments of the E1a regions exhibit higher than average homology, for both coding and noncoding sequences. The latter may specify the initiation of DNA replication, DNA encapsidation and regulation of expression. The predicted polypeptides encoded by the E1a regions of the three serotypes exhibit a large degree of homology.