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. 1981 Jan;37(1):48-54.
doi: 10.1128/JVI.37.1.48-54.1981.

Pichinde virus L and S RNAs contain unique sequences

W C Leung, A Ramsingh, K Dimock, W E Rawls, J Petrovich, M Leung

Pichinde virus L and S RNAs contain unique sequences

W C Leung et al. J Virol. 1981 Jan.

Abstract

Using oligodeoxyribonucleotides produced by limited DNase I digestion of calf thymus DNA as a primer, we synthesized complementary DNA (cDNA) from the L and the S RNAs of Pichinde virus. The reaction conditions for in vitro cDNA synthesis were optimized to allow transcription of about 90% of either L or S RNA. No significant hybridization was observed when the L cDNA was hybridized to the S RNA, or when the S cDNA was hybridized to the L RNA. The results indicate that the L and S RNAs of Pichinde virus contain unique nucleotide sequences.

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References

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