doi: 10.1016/0304-4165(81)90468-2.
Early events in guinea pig reticulocyte iron uptake
- PMID: 7225422
- DOI: 10.1016/0304-4165(81)90468-2
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Early events in guinea pig reticulocyte iron uptake
Biochim Biophys Acta.
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Abstract
Hemolysates, prepared from guinea pig reticulocytes incubated with 59Fe-labelled serum, can be resolved into five peaks utilizing molecular sieve chromatography: ferritin, transferrin, hemoglobin, an Mx 17 000 fraction, and a low molecular weight fraction. The hemoglobin peak also contains a nonhemoglobin component (III-X), demonstrated by heme extraction and by isoelectric focusing. Transferrin, the III-X component and the low molecular weight fraction are the first to accumulate radioactive iron during the reticulocyte incubation. The 59Fe in each of these also chases. Therefore, a role for these components as precursors to iron incorporation into heme is suggested.
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