Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Feb;33(2):275-9.
doi: 10.1016/S0006-3495(81)84889-8.

On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin

A G Doukas, A Pande, T Suzuki, R H Callender, B Honig, M Ottolenghi

On the mechanism of hydrogen-deuterium exchange in bacteriorhodopsin

A G Doukas et al. Biophys J. 1981 Feb.

Abstract

Continuous-flow resonance Raman experiments carried out in bacteriorhodopsin show that the exchange of a deuteron on the Schiff base with a proton takes place in times shorter than 3 ms. Exchange mechanisms based on a base-catalyzed deprotonation followed by reprotonation of the Schiff base are excluded. A mechanism is suggested in which a water molecule interacts directly with the Schiff base deuteron in a concerted exchange mechanism. It appears that in the dark, the binding site is more accessible to neutral water molecules than to charged protons.

PubMed Disclaimer

References

    1. Annu Rev Biochem. 1972;41:903-24 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4462-6 - PubMed
    1. Prep Biochem. 1975;5(2):161-78 - PubMed
    1. Biochemistry. 1976 Apr 20;15(8):1621-9 - PubMed
    1. Biochemistry. 1976 Oct 19;15(21):4593-9 - PubMed

Publication types

LinkOut - more resources