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. 1981 Feb 25;9(4):879-94.
doi: 10.1093/nar/9.4.879.

The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA

R B Wallace, M J Johnson, T Hirose, T Miyake, E H Kawashima, K Itakura
Free PMC article

The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA

R B Wallace et al. Nucleic Acids Res. .
Free PMC article

Abstract

Two oligonucleotides 14-bases long were synthesized, one complementary to rabbit beta-globin DNA (R beta G14A) and the other with the same sequence except for a single base change (T for C) (R beta G14B). Hybridization conditions were established such that R beta G14A would hybridize to globin DNA while R beta G14B would not. We also synthesized a mixture of 13-base long oligonucleotides (R beta G13Mix), representing eight of the possible coding sequences for amino acids 15-19 of rabbit beta-globin. One of the eight is complementary to globin DNA. R beta G13Mix was found to hybridize specifically to globin DNA under conditions where oligonucleotides forming single base pair mismatches do not. Furthermore, R beta G13Mix was shown to hybridize specifically to colonies containing a plasmid with a globin DNA insert. These results are discussed with respect to a general procedure for screening recombinant clones for those containing DNA coding for a protein of known amino acid sequence.

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References

    1. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
    1. Nucleic Acids Res. 1980 Nov 25;8(22):5491-505 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 - PubMed
    1. Science. 1977 Apr 8;196(4286):161-9 - PubMed

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