Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Apr 10;9(7):1643-56.
doi: 10.1093/nar/9.7.1643.

ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA

N Sonenberg
Free PMC article

ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA

N Sonenberg. Nucleic Acids Res. .
Free PMC article

Abstract

Two proteins of apparent molecular weights of 28,000 and 50,000 daltons were shown to recognize and cross-link specifically to the 5' cap end of oxidized reovirus mRNA. Cross-linking of these proteins to mRNA was ATP/Mg++ dependent, in sharp contrast to cross-linking of a 24K cap binding protein which was purified and characterized previously (Sonenberg, N., Rupprecht, K.M., Hecht, S.M. and Shatkin, A.J. (1979) Proc. Natl. Acad. Sci, USA 76, 4345-4349). Non-hydrolyzable analogues of ATP as well as other nucleotides did not substitute for ATP in the cross-linking reaction and Mg++ was significantly preferred over other divalent cations in cross-linking of the 28K and 50K dalton proteins. A model involving the function of the latter proteins in recognition and unwinding of the 5' end structure of capped eukaryotic mRNAs is suggested.

PubMed Disclaimer

References

    1. J Biol Chem. 1970 Mar 10;245(5):955-61 - PubMed
    1. Nature. 1980 May 29;285(5763):331-3 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Feb;73(2):375-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1834-8 - PubMed