Vagally innervated and denervated gastric pouch in one and the same dog--a new model

Abstract

A new model for studies of gastric secretion is introduced. Two pouches separated from the stomach are constructed in one and the same dog. A vagally denervated pouch is taken from the corporo-fundic part of the stomach, and a vagally innervated pouch is constructed from a pear-shaped piece of the anterior wall with basis in the corporeal part of the lesser curvature. Intact vagal innervation is tested with 2-deoxy-D-glucose. The maximal histamine response is used as reference value for each pouch. A dose-response study for histamine stimulation is presented. The model has proved to be surgically well reproducible: of the first 16 dogs operated upon, 12 have shown appropriate magnitude of secretion. The dogs can live for months and years; a tendency to hypopotassaemia is easily normalized by oral KCl.