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. 1981 Feb;41(2):545-8.
doi: 10.1128/aem.41.2.545-548.1981.

Method for measuring dissolved hydrogen in anaerobic ecosystems: application to the rumen

J A Robinson, R F Strayer, J M Tiedje

Method for measuring dissolved hydrogen in anaerobic ecosystems: application to the rumen

J A Robinson et al. Appl Environ Microbiol. 1981 Feb.

Abstract

A method of transferring dissolved H2 to a CO2 headspace and then absorbing out the CO2 to concentrate the H2 before gas chromatographic analysis was developed to measure low concentrations of dissolved H2. A detection limit of 10 pmol of H2 ml-1 of water was achieved. When used ot monitor H2 changes in a bovine rumen, a 10-fold increase in H2 was noted 1 h after feeding and then declined rapidly to the normal steady-state concentration of 1 microM.

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