Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Apr;43(4):443-50.
doi: 10.1038/bjc.1981.65.

In vitro metabolism of misonidazole

P D Josephy, B Palcic, L D Skarsgard
Free PMC article

In vitro metabolism of misonidazole

P D Josephy et al. Br J Cancer. 1981 Apr.
Free PMC article

Abstract

Misonidazole (MISO) is a nitroimidazole drug currently undergoing clinical trials as a radiosensitizer of hypoxic tumour cells. The drug is also toxic to such cells, probably because of metabolic activation of the nitro group under hypoxia. The metabolic fate of 14C-labelled MISO is examined, using hypoxic mammalian (CHO) cells in vitro. Organic-soluble and acid-soluble metabolites are formed, and radioactivity is bound to macromolecules. The organic-soluble products are separated by TLC and HPLC. Evidence is presented to show that one of the metabolites is hydroxylamino-misonidazole. The significance of metabolic nitroreduction is discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochemistry. 1975 Apr 22;14(8):1626-32 - PubMed
    1. Cancer Clin Trials. 1980 Fall;3(3):237-51 - PubMed
    1. Cancer Res. 1976 Oct;36(10):3761-5 - PubMed
    1. Radiat Res. 1976 Sep;67(3):459-73 - PubMed
    1. Curr Top Radiat Res Q. 1977 Aug;11(4):347-98 - PubMed

Publication types