doi: 10.1093/oxfordjournals.jbchem.a133207.
A new method for the preparation of acyl-CoA thioesters
- PMID: 7240117
- DOI: 10.1093/oxfordjournals.jbchem.a133207
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A new method for the preparation of acyl-CoA thioesters
J Biochem.
1981 Feb.
Free article
Abstract
A method is described for the preparation of CoA thioesters of fatty acids. Acyl-CoA thioester was synthesized by way of 1-acylimidazole and purified by high performance liquid chromatography. This method is applicable to the preparation of a variety of acyl-CoA thioesters of various chain lengths (from C2 to C18) and with varying degrees of unsaturation. It is particularly suitable for preparing acyl-CoA thioesters of labeled fatty acids or fatty acids available only in small amounts.
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