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. 1981 Feb;89(2):337-9.
doi: 10.1093/oxfordjournals.jbchem.a133207.

A new method for the preparation of acyl-CoA thioesters

A Kawaguchi, T Yoshimura, S Okuda
Free article

A new method for the preparation of acyl-CoA thioesters

A Kawaguchi et al. J Biochem. 1981 Feb.
Free article

Abstract

A method is described for the preparation of CoA thioesters of fatty acids. Acyl-CoA thioester was synthesized by way of 1-acylimidazole and purified by high performance liquid chromatography. This method is applicable to the preparation of a variety of acyl-CoA thioesters of various chain lengths (from C2 to C18) and with varying degrees of unsaturation. It is particularly suitable for preparing acyl-CoA thioesters of labeled fatty acids or fatty acids available only in small amounts.

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