The molecular structure of lubricating glycoprotein-I, the boundary lubricant for articular cartilage
- PMID: 7240180
The molecular structure of lubricating glycoprotein-I, the boundary lubricant for articular cartilage
Abstract
Lubricating glycoprotein-I (LGP-I) was prepared from bovine synovial fluid by density gradient sedimentation and gel-permeation chromatography. The LGP-I sample obtained was able to lubricate articular cartilage in a manner equivalent to that of whole synovial fluid. Chemical, physical, and electron microscope measurements were carried out to determine the structure of the LGP-I molecules. The molecular weight calculated from sedimentation equilibrium measurements was 2 X 10(5), and the solute distribution obtained indicated that LGP-I was relatively monodisperse. The s(0)20,w value was 4.84, and the intrinsic viscosity was 92 ml/g. The molecular weight and diffusion coefficient calculated from later light-scattering measurements was 2.06 x 10(5) and 1.10 x 10(-7) cm2/s, respectively. The electron microscope measurements showed that the LGP-I molecules had a number average length of 204 nm, a weight average length of 222 nm (with a standard deviation of 54 nm), and a width of 1-2 nm. These data and the kinked appearance of the molecules indicate that LGP-I is a partially extended flexible rod. The hydrodynamic measurements also indicate that LGP-I has the same structure in solution, although the apparently high s(0)20,w value, compared to other rod-like molecules, suggests that due to its flexibility LGP-I can occupy a more compact domain than would be expected based on its extended dimensions. The name of "lubricin" is suggested for this lubricating glycoprotein.
Similar articles
-
The isolation and properties of a second glycoprotein (LGP-II) from the articular lubricating fraction from bovine synovial fluid.Biochem J. 1979 Jun 1;179(3):465-71. doi: 10.1042/bj1790465. Biochem J. 1979. PMID: 112997 Free PMC article.
-
The isolation and partial characterization of the major glycoprotein (LGP-I) from the articular lubricating fraction from bovine synovial fluid.Biochem J. 1977 Mar 1;161(3):473-85. doi: 10.1042/bj1610473. Biochem J. 1977. PMID: 15548 Free PMC article.
-
The lubricating activity of synovial fluid glycoproteins.Arthritis Rheum. 1981 Jan;24(1):22-30. doi: 10.1002/art.1780240104. Arthritis Rheum. 1981. PMID: 7470168
-
[Influence of Synovial Fluid on Lubrication of Articular Cartilage in Vitro - A Review].Z Orthop Unfall. 2018 Apr;156(2):205-213. doi: 10.1055/s-0043-117959. Epub 2017 Dec 12. Z Orthop Unfall. 2018. PMID: 29232729 Review. German.
-
Lubrication of Articular Cartilage.Annu Rev Biomed Eng. 2016 Jul 11;18:235-58. doi: 10.1146/annurev-bioeng-081514-123305. Annu Rev Biomed Eng. 2016. PMID: 27420572 Review.
Cited by
-
The distribution of superficial zone protein (SZP)/lubricin/PRG4 and boundary mode frictional properties of the bovine diarthrodial joint.J Biomech. 2015 Sep 18;48(12):3406-12. doi: 10.1016/j.jbiomech.2015.05.032. Epub 2015 Jun 12. J Biomech. 2015. PMID: 26117076 Free PMC article.
-
The role of the non-collagenous matrix in tendon function.Int J Exp Pathol. 2013 Aug;94(4):248-59. doi: 10.1111/iep.12027. Epub 2013 May 30. Int J Exp Pathol. 2013. PMID: 23718692 Free PMC article. Review.
-
Quantitative proteomic analysis of eight cartilaginous tissues reveals characteristic differences as well as similarities between subgroups.J Biol Chem. 2012 Jun 1;287(23):18913-24. doi: 10.1074/jbc.M111.298968. Epub 2012 Apr 9. J Biol Chem. 2012. PMID: 22493511 Free PMC article. Clinical Trial.
-
In vivo efficacy of fresh versus frozen osteochondral allografts in the goat at 6 months is associated with PRG4 secretion.J Orthop Res. 2013 Jun;31(6):880-6. doi: 10.1002/jor.22319. Epub 2013 Jan 29. J Orthop Res. 2013. PMID: 23362152 Free PMC article.
-
Multimodal evaluation of tissue-engineered cartilage.J Med Biol Eng. 2013 Feb 1;33(1):1-16. doi: 10.5405/jmbe.1254. J Med Biol Eng. 2013. PMID: 23606823 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources