TLC identification adn GLC determination of meperidine and its metabolites in biological fluids
- PMID: 7241346
- DOI: 10.1002/jps.2600700504
TLC identification adn GLC determination of meperidine and its metabolites in biological fluids
Abstract
Procedures were developed for TLC identification and GLC determination of meperidine and its metabolites, i.e., p-hydroxymeperidine, normeperidine, and meperidinic and normeperidinic acids. Meperidine, p-hydroxymeperidine, and normeperidine were extracted with ether from biological fluids at pH 10, whereas meperidinic and normeperidinic acids and conjugated metabolites remained in the aqueous phase. The residue, upon evaporation of the extract to dryness, was derivatized with trifluoroacetic anhydride and gas chromatographed. Total (free and conjugated) meperidinic and normeperidinic acids in the aqueous phase were converted and determined as meperidine and normeperidine, respectively. A preliminary result of urinary disposition of meperidine and its metabolites in the rat is presented. The identity of these metabolites was confirmed with GLC-mass spectrometry.
Similar articles
-
GLC determination of meperidinic and normeperidinic acids in urine.J Pharm Sci. 1978 Jan;67(1):116-8. doi: 10.1002/jps.2600670131. J Pharm Sci. 1978. PMID: 619098
-
Urinary excretion of meperidine and its metabolites.J Pharm Sci. 1981 Aug;70(8):867-70. doi: 10.1002/jps.2600700808. J Pharm Sci. 1981. PMID: 7310653
-
Investigation of meperidine and its metabolites in urine of an addict by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry.J Chromatogr B Biomed Appl. 1994 Aug 19;658(2):375-9. doi: 10.1016/0378-4347(94)00240-1. J Chromatogr B Biomed Appl. 1994. PMID: 7820266
-
Detection of bacterial metabolites in spent culture media and body fluids by electron capture gas-liquid chromatography.Adv Chromatogr. 1977;15:1-31. Adv Chromatogr. 1977. PMID: 320833 Review.
-
Analysis of insecticide synergists.J Chromatogr Sci. 1975 May;13(5):238-45. doi: 10.1093/chromsci/13.5.238. J Chromatogr Sci. 1975. PMID: 49362 Review.
Cited by
-
Clinical pharmacokinetics of pethidine: 1982.Clin Pharmacokinet. 1982 Sep-Oct;7(5):421-33. doi: 10.2165/00003088-198207050-00003. Clin Pharmacokinet. 1982. PMID: 6754208 Review. No abstract available.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous