Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 May;70(5):482-6.
doi: 10.1002/jps.2600700504.

TLC identification adn GLC determination of meperidine and its metabolites in biological fluids

TLC identification adn GLC determination of meperidine and its metabolites in biological fluids

S Y Yeh et al. J Pharm Sci. 1981 May.

Abstract

Procedures were developed for TLC identification and GLC determination of meperidine and its metabolites, i.e., p-hydroxymeperidine, normeperidine, and meperidinic and normeperidinic acids. Meperidine, p-hydroxymeperidine, and normeperidine were extracted with ether from biological fluids at pH 10, whereas meperidinic and normeperidinic acids and conjugated metabolites remained in the aqueous phase. The residue, upon evaporation of the extract to dryness, was derivatized with trifluoroacetic anhydride and gas chromatographed. Total (free and conjugated) meperidinic and normeperidinic acids in the aqueous phase were converted and determined as meperidine and normeperidine, respectively. A preliminary result of urinary disposition of meperidine and its metabolites in the rat is presented. The identity of these metabolites was confirmed with GLC-mass spectrometry.

PubMed Disclaimer

Similar articles

Cited by

  • Clinical pharmacokinetics of pethidine: 1982.
    Edwards DJ, Svensson CK, Visco JP, Lalka D. Edwards DJ, et al. Clin Pharmacokinet. 1982 Sep-Oct;7(5):421-33. doi: 10.2165/00003088-198207050-00003. Clin Pharmacokinet. 1982. PMID: 6754208 Review. No abstract available.

LinkOut - more resources