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. 1981 Mar-Apr;17(2):247-53.

[Modified method for producing ribonuclease A from amorphous and crystalline ribonucleases]

[Article in Russian]
Iu O Lebedev, S V Shliapnikov, S N Borisova, L V Abaturov
  • PMID: 7243747

[Modified method for producing ribonuclease A from amorphous and crystalline ribonucleases]

[Article in Russian]
Iu O Lebedev et al. Prikl Biokhim Mikrobiol. 1981 Mar-Apr.

Abstract

The paper describes a method for producing gram-scale quantities of beef pancreatic ribonuclease A (RNase A) (EC 3.1.4.22) from commercial, amorphous and crystalline RNases. The method involves enzyme purification by chromatography on SP-Sephadex G-25, rechromatography on Bio-Rax 70 followed by concentration, storage and desalting in optimal conditions. In their properties the resultant preparations of RNase A are not inferior to the best commercial samples of the enzyme.

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