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. 1978 Nov;5(11):4105-27.
doi: 10.1093/nar/5.11.4105.

A computer assisted method for the determination of restriction enzyme recognifion sites

T R Gingeras, J P MIlazzo, R J Roberts
Free PMC article

A computer assisted method for the determination of restriction enzyme recognifion sites

T R Gingeras et al. Nucleic Acids Res. 1978 Nov.
Free PMC article

Abstract

A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has been tested using fragmentation patterns of 0X174 DNA produced by enzymes of both known and unknown specificity. Recognition sequences are predicted for two restriction endonucleases (BbvI and SfaNI) using this method. In addition, recognition sequences are predicted for two other new enzymes (PvuI and MstI) using another computer-assisted method.

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