[Inhibitory analysis of the luminescent electron transport chain of Photobacterium fischeri]

Abstract

The quenching of luminescence of bacterial luciferase from Photobacterium fischeri by non-specific electron acceptors and inhibitors of dehydrogenases was studied. The inhibition of the luminescent reaction obeys the non-competitive mechanism with NADH, FMN and aliphatic aldehyde. The inhibitors compete with cytochrome c for NADH -- cytochrome c oxido-reductase. It is concluded that lumiredoxin, a FeS-containing protein, is the most sensitive component of the luminescent electron transport chain.