doi: 10.1007/BF00210087.
Isolation and primary culture of adult human hepatocytes. Ultrastructural and functional studies
- PMID: 7249058
- DOI: 10.1007/BF00210087
Item in Clipboard
Isolation and primary culture of adult human hepatocytes. Ultrastructural and functional studies
Cell Tissue Res.
1981.
Abstract
Biopsy tissue of adult human liver was gently dissociated with collagenase followed by Dispase. By repeated low g centrifugation, a large number of almost pure, viable hepatocytes was obtained. This is the first report of a successful procedure for obtaining adult human hepatocytes for study in tissue culture. The isolated cells have the typical morphology of liver parenchyma, and these characteristics persist throughout the period of culturing. Evidence of their function is indicated by albumin synthesis. This procedure is now being used to study human hepatocyte functions in vitro and the effects of a variety of agents including carcinogens and viruses.
Similar articles
-
Isolation and long-term maintenance of differentiated adult chicken hepatocytes in primary culture.In Vitro Cell Dev Biol. 1992 Sep-Oct;28A(9-10):615-20. doi: 10.1007/BF02631036. In Vitro Cell Dev Biol. 1992. PMID: 1429363
-
Functional human hepatocytes: isolation from small liver biopsy samples and primary cultivation with liver-specific functions.J Toxicol Sci. 1995 Nov;20(5):565-78. doi: 10.2131/jts.20.5_565. J Toxicol Sci. 1995. PMID: 8720163
-
Isolation of rat hepatocytes with EDTA and their metabolic functions in primary culture.In Vitro Cell Dev Biol. 1985 Sep;21(9):526-30. doi: 10.1007/BF02620846. In Vitro Cell Dev Biol. 1985. PMID: 2995304
-
[Cell culture and its application primary culture of human hepatocytes and its application].Gan To Kagaku Ryoho. 1992 Aug;19(9):1411-9. Gan To Kagaku Ryoho. 1992. PMID: 1503493 Review. Japanese.
-
Isolated mammalian hepatocytes in culture.Gastroenterology. 1976 Dec;71(6):1086-96. Gastroenterology. 1976. PMID: 11143 Review. No abstract available.
Cited by
-
Fine structure and function of isolated gonadotropic cells as revealed from pituitaries of immature rainbow trout, Salmo gairdneri, by means of a new enzymatic dispersion technique.Cell Tissue Res. 1982;226(3):641-53. doi: 10.1007/BF00214791. Cell Tissue Res. 1982. PMID: 6291764
-
Effect of chenodeoxycholic and ursodeoxycholic acids on isolated adult human hepatocytes.Dig Dis Sci. 1984 Dec;29(12):1123-30. doi: 10.1007/BF01317087. Dig Dis Sci. 1984. PMID: 6499631
-
Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells.Development. 2016 May 1;143(9):1475-81. doi: 10.1242/dev.132209. Development. 2016. PMID: 27143754 Free PMC article.
-
Demonstration and maintenance of mucus secretion in cultured human gallbladder epithelial cells.In Vitro Cell Dev Biol. 1987 Aug;23(8):559-66. doi: 10.1007/BF02620973. In Vitro Cell Dev Biol. 1987. PMID: 3624158
-
Attachment and multiplication, morphology and protein production of human fetal primary liver cells cultured in hormonally defined media.In Vitro Cell Dev Biol. 1988 Mar;24(3):230-8. doi: 10.1007/BF02623552. In Vitro Cell Dev Biol. 1988. PMID: 2450864