doi: 10.1016/s0021-9673(00)81590-3.
Application of simultaneous UV-radioactivity high-performance liquid chromatography to the study of intermediary metabolism. I. Purine nucleotides, nucleosides and bases
- PMID: 7251721
- DOI: 10.1016/s0021-9673(00)81590-3
Item in Clipboard
Application of simultaneous UV-radioactivity high-performance liquid chromatography to the study of intermediary metabolism. I. Purine nucleotides, nucleosides and bases
J Chromatogr.
.
Abstract
Procedures are presented for the continuous-flow, simultaneous measurement of concentration and radioactivity of purine metabolites separated by high-performance liquid chromatography (HPLC). A microprocessor-controlled radioactivity flow detector connected in series to a UV-flow detector provides on-line quantitative monitoring of separated components in the post-column effluent stream. Through use of two HPLC separations--reversed-phase and anion-exchange--quantitation of all major purine nucleotides, nucleosides and bases is possible. The procedures provides a rapid, sensitive, and convenient means for the systematic study of purine metabolism.
Similar articles
-
Analysis of radioactive and nonradioactive purine bases, purine nucleosides and purine nucleotides by high-speed chromatography on a single column.Monogr Hum Genet. 1978;10:127-34. doi: 10.1159/000401581. Monogr Hum Genet. 1978. PMID: 723885 No abstract available.
-
Analysis of radioactive and nonradioactive purine bases, nucleosides, and nucleotides by high-speed chromatography on a single column.Anal Biochem. 1978 May;86(1):65-77. doi: 10.1016/0003-2697(78)90319-6. Anal Biochem. 1978. PMID: 655401 No abstract available.
-
Simultaneous determination of myocardial nucleotides, nucleosides, purine bases and creatine phosphate by ion-pair high-performance liquid chromatography.J Chromatogr. 1992 Jul 1;578(1):39-44. doi: 10.1016/0378-4347(92)80222-c. J Chromatogr. 1992. PMID: 1400784
-
Reversed-phase high-performance liquid chromatography of purine compounds for investigation of biomedical problems: application to different tissues and body fluids.J Chromatogr. 1993 Aug 25;618(1-2):15-40. doi: 10.1016/0378-4347(93)80025-y. J Chromatogr. 1993. PMID: 8227254 Review.
-
Current state of the art in the HPLC analyses of free nucleotides, nucleosides, and bases in biological fluids.Adv Chromatogr. 1980;18:101-38. Adv Chromatogr. 1980. PMID: 6994448 Review.
Cited by
-
Purine metabolism in myeloid precursor cells during maturation. Studies with the HL-60 cell line.J Clin Invest. 1983 Dec;72(6):1889-900. doi: 10.1172/JCI111152. J Clin Invest. 1983. PMID: 6139386 Free PMC article.
-
Antimalarial properties of bredinin. Prediction based on identification of differences in human host-parasite purine metabolism.J Clin Invest. 1982 Aug;70(2):461-9. doi: 10.1172/jci110636. J Clin Invest. 1982. PMID: 7047569 Free PMC article.
-
In vitro effects of mycophenolic acid and allopurinol against Leishmania tropica in human macrophages.Antimicrob Agents Chemother. 1982 Jun;21(6):887-91. doi: 10.1128/AAC.21.6.887. Antimicrob Agents Chemother. 1982. PMID: 7114837 Free PMC article.
-
HPLC analysis of organochlorines using UV and radioactivity flow detectors.Bull Environ Contam Toxicol. 1984 Mar;32(3):301-9. doi: 10.1007/BF01607502. Bull Environ Contam Toxicol. 1984. PMID: 6713123 No abstract available.