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. 1981 Apr 10;223(1):131-8.
doi: 10.1016/s0378-4347(00)80075-4.

High-performance liquid chromatographic methods for determination of gentamicin in biological fluids

S E Walker, P E Coates

High-performance liquid chromatographic methods for determination of gentamicin in biological fluids

S E Walker et al. J Chromatogr. .

Abstract

A selective and sensitive methods for the determination of gentamicin in plasma and urine by high-performance liquid chromatography has been developed. Following deproteinization, the gentamicin is reacted with fluorescamine to produce a fluorescent derivative. This reaction mixture is directly chromatographed on a cation exchange column using as mobile phase acetonitrile-phosphoric acid (7:3). The gentamicin compounds elute as a single peak. Using 0.1 ml of plasma, quantitation of gentamicin concentration as low as 1 mg/l are possible. Possible interference from other aminoglycosides and antibiotics is discussed.

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