doi: 10.1016/0005-2736(77)90052-9.
NMR observation of gramicidin A' in phosphatidylcholine vesicles
- PMID: 72565
- DOI: 10.1016/0005-2736(77)90052-9
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NMR observation of gramicidin A' in phosphatidylcholine vesicles
Biochim Biophys Acta.
.
Abstract
Dimyristoyl phosphatidylcholine was prepared with perdeuterated hydrocarbon chains and sonicated into bilayer vesicles together with gramicidin A'. The 1H NMR resonance from the tryptophan residues in the gramicidin has a linewidth of approximately 80 Hz, indicating significant local mobility for these residues. Paramagnetic lanthanides added to the aqueous medium cause a chemical shift of this signal indicating that some of the tryptophans may be located in the interfacial region of the bilayer.
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