[Quantitative kinetic study of the histochemical reaction to peroxidase in salivary gland and uterine cells of golden hamsters]

Abstract

The kinetics of diaminobenzidine reaction for peroxidase has been studied using cryostat sections of salivary gland and uterus of the golden hamster. It was demonstrated that the inhibition of the initial velocity of the reaction in salivary gland occurred at hydrogen peroxide concentration in the reaction medium being 5.10(-3) M or higher. On the other hand, the inhibitory action of hydrogen peroxide excess on the uterine peroxidase activity was observed at H2O2 concentrations higher than 1.10(-3) M. KM values for diaminobenzidine, determined for salivary gland and uterine peroxidases, were 2.3.10(-3) M and 3.5.10(-3) M, resp; KM values for H2O2 were 1.1.10(-4) M and 2.5.10(-5) M, resp. Kinetic difference of salivary gland and uterine peroxidases obtained in this study support the view on the organ specificity of peroxidases.