[Nucleotide conformation in aqueous solutions by the NMR spectrum lanthanide shift method]

Abstract

Nucleoside triphosphates, ATP in particular, are investigated by 1H and 31P NMR with lanthanide perchlorate as LSR. The nucleotide base is shown to be near the phosphate groups. Dinucleotides and nucleoside monophosphates are investigated by means of a new LSR--lanthanide complex of pirydoxalidenaspartic acid. NAD+ and its derivatives are shown to exist in folded conformation at high pH values. AMP is shown to undergo conformational change in the pH range between 7 and 8.5. The structure of adduct of new LSR with amino acid is calculated in assumption of unaxial symmetry of the magnetic dipolar interaction. Parameters of the interaction were determined from optical spectra.