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. 1981;100(2):225-8.
doi: 10.1007/BF00403369.

[Possibilities and limits of the quantitative demonstration of protein thiols with the DDD-reagent in cells of cervical epithelium (author's transl)]

[Article in German]
E Schauentstein, G Nöhammer, F Bajardi, W Urdl

[Possibilities and limits of the quantitative demonstration of protein thiols with the DDD-reagent in cells of cervical epithelium (author's transl)]

[Article in German]
E Schauentstein et al. J Cancer Res Clin Oncol. 1981.

Abstract
in English, German

From six patients, all with invasive carcinoma of the cervix uteri, two groups fo smear preparations, each consisting of eight samples, were taken: The first, group A, before and the second, group B, after mechanical removal of the discharge which was present in abundant quantities. All samples were treated for 24 h with DDD to reach the fast-reaching SH-groups, coupled with Fast Blue B and measured cytophotometrically at 560 nm. Without any exception, the neoplastic cells of group A showed significantly lower extinction values both of the nucleus and of the total cell, if compared to group B. The mean extinction difference amounts to 55% and is highly significant and some evidence is given that the viability of the cells plays an important role. For this reason, the quantitative evaluation of the DDD Fast Blue B does not seem to be useful for cytologic routine investigations.

Von sechs Patientinnen mit invasiven Portio-Carcinom wurden je acht Abstriche vor Entfernung des reichlich vorhandenen Fluors (Gruppe A) und weitere acht Abstriche nach mechanischer Säuberung der Portio entnommen (Gruppe B). Sämtliche Präparate wurden 24 h mit DDD und Echtblau B auf schnell reagierende Proteinthiole gefärbt und photometriert. Die neoplastischen Zellen der Gruppe A zeigten ohne Ausnahme hochsignifikant geringere Extinktionen des Kerns und der Gesamtzelle im Vergleich zur gruppe B.

Die Extinktionsdifferenz von durchschnittlich 55% ist hoch signifikant. Der Vitalzustand der Zellen spielt in diesem Zusammenhang offensichtlich eine wichtige Rolle. Für cytologische Routineuntersuchungen erscheint daher die quantitative Auswertung der DDD-Echtblau B-Färbung nicht geeignet.

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References

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