doi: 10.1016/0022-1759(81)90020-x.
Improved method for the isolation of purified mouse peritoneal macrophages
- PMID: 7264322
- DOI: 10.1016/0022-1759(81)90020-x
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Improved method for the isolation of purified mouse peritoneal macrophages
J Immunol Methods.
1981.
Abstract
Mouse peritoneal macrophages were cultured for 45 min in medium supplemented with fetal calf serum (FCS) is petri dishes coated overnight with heat-inactivated FCS. After removal of non-adherent cells by washing, adherent cells were detached by a brief incubation in the presence of sub-toxic levels of ethylenediamine tetraacetate (EDTA). Overall peritoneal macrophage recoveries of 90% can be routinely achieved with this method, and full cell viability is maintained.
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