doi: 10.1016/0165-7992(81)90006-3.
Quantitative estimation of the extent of alkylation of DNA following treatment of mammalian cells with non-radioactive alkylating agents
- PMID: 7266573
- DOI: 10.1016/0165-7992(81)90006-3
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Quantitative estimation of the extent of alkylation of DNA following treatment of mammalian cells with non-radioactive alkylating agents
Mutat Res.
1981 Jul-Sep.
Abstract
Alkaline sucrose sedimentation has been used to quantitate phosphotriester formation following treatment of human cells with the monofunctional alkylating agents methyl and ethyl methanesulfonate. These persistent alkaline-labile lesions are not repaired during short-term culture conditions and thus serve as a useful and precise index of the total alkylation of the DNA. Estimates of alkylation by this procedure compare favorably with direct estimates by use of labeled alkylating agents.
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