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. 1981 Aug;38(3):304-6.
doi: 10.1136/oem.38.3.304.

Thin-layer chromatography of hippuric and m-methylhippuric acid in urine after mixed exposure to toluene and xylene

G Bieniek, T Wilczok

Thin-layer chromatography of hippuric and m-methylhippuric acid in urine after mixed exposure to toluene and xylene

G Bieniek et al. Br J Ind Med. 1981 Aug.

Abstract

The separation of hippuric and m-methylhippuric acid as toluene and m-xylene metabolites present in urine of people exposed simultaneously to toluene and xylene is described. Chloroform was used for hippuric and m-methylhippuric acid extraction. Satisfactory separation of these metabolites was obtained on TLC plates covered with silica gels and developed in chloroform acetic acid-water (4:1:1);p-dimethylaminobenzaldehyde in acetic acid anhydride was applied to develop the colour. The sensitivity of the method was 6 micrograms hippuric acid per 1 ml urine and recovery was 100% (+/- 1).

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