Chromosomes and causation of human cancer and leukemia. XLIV. A method for chromosome analysis of solid tumors

Abstract

Mechanical and enzymatic disaggregation procedures have been utilized for the preparation of materials suitable for chromosome examination of tissues from various kinds of solid tumors. A great number of the cells disaggregated with the latter procedure were observed to attach to the bottom of culture flasks after 2 days in vitro; cells obtained with the former method were not attached. In general, the enzymatic procedure yielded a significantly larger number of viable cells that would undergo mitosis after short term culture and considerably improved the quality of banding as compared to the mechanical method. However, there were no significant differences among the basic karyotypes observed in the cells obtained by the mechanical approach versus those seen following the enzymatic method. This evidence, together with the high success rate of karyotyping in the enzyme-treated preparations, suggest that this should be the procedure of preference for cytogenetic examination of various tumors, particularly where in the past karyotypic examination has been very difficult, e.g., the early stages of tumor development.