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. 1981;45(2):109-15.
doi: 10.1016/0022-1759(81)90205-2.

Estimation of the number of monoclonal hybridomas in a cell fusion experiment. Effect of post-fusion cell dilution on hybridoma survival

A L De Blas, M V Ratnaparkhi, J E Mosimann

Estimation of the number of monoclonal hybridomas in a cell fusion experiment. Effect of post-fusion cell dilution on hybridoma survival

A L De Blas et al. J Immunol Methods. 1981.

Abstract

A practical method is described for the estimation of the number of monoclonal hybridomas in a cell fusion experiment as a function of the percent of culture dishes showing hybridoma growth. Our method is based on the Poisson probability model. A justification for the method is included. The application of this model to our experimental results indicates that the probability of hybridoma survival decreases with post-fusion cell dilution even in the presence of a constant number of feeder cells.

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