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. 1981 Oct;62(5):469-73.

Some cytofluorimetric studies of the nuclear ploidy of mouse hepatocytes. I. A simple method for isolation of hepatocyte nuclei using in situ perfusion of the liver

P R Steele, A P Yim, B M Herbertson, J Watson

Some cytofluorimetric studies of the nuclear ploidy of mouse hepatocytes. I. A simple method for isolation of hepatocyte nuclei using in situ perfusion of the liver

P R Steele et al. Br J Exp Pathol. 1981 Oct.

Abstract

A simple method for isolating mouse hepatocyte nuclei is presented, which requires no special apparatus. Flow cytofluorimetric scans of hepatocyte nuclei, prepared by this method and stained with ethidium bromide, showed that adult mice have a high proportion of their hepatocyte nuclei in the tetraploid and octaploid states. Polyploidy was more pronounced in female animals.

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