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. 1981;170(1):37-43.
doi: 10.1007/BF02123795.

Stabilization of adenovirus infectivity against thermoinactivation by lowered ionic strength

G Sprunck, R Wigand

Stabilization of adenovirus infectivity against thermoinactivation by lowered ionic strength

G Sprunck et al. Med Microbiol Immunol. 1981.

Abstract

The thermostability of adenovirus 5 increases when the ionic strength is decreased before heating by dilution in distilled water (1 : 100, heating at 50 degrees C). A predilution in Eagle's MEM before heating or a previous dialysis against water had no effect on thermostability. The stabilizing effect was found likewise in crude and purified virus, in heating at 50 degrees C up to 320 min, from 50 degrees C to 56 degrees C, and in dilution from 10(-2) to 10(-5). The degree of stabilization was similar to cationic stabilization in 2M NaCl. The infectivity of heated adenovirus 5 was not sensitivity to DNase digestion. Other adenovirus types were stabilized in a similar manner.

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References

    1. J Gen Virol. 1967 Oct;1(4):553-60 - PubMed
    1. Virology. 1969 Nov;39(3):599-604 - PubMed
    1. J Gen Virol. 1967 Oct;1(4):509-22 - PubMed
    1. J Immunol. 1962 Jul;89:41-6 - PubMed
    1. J Virol. 1977 Mar;21(3):1159-69 - PubMed