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. 1981 Nov;14(5):496-500.
doi: 10.1128/jcm.14.5.496-500.1981.

Rapid and direct staphylocoagulase assay that uses a chromogenic substrate for identification of Staphylococcus aureus

W Engels, M A Kamps, C P van Boven

Rapid and direct staphylocoagulase assay that uses a chromogenic substrate for identification of Staphylococcus aureus

W Engels et al. J Clin Microbiol. 1981 Nov.

Abstract

A rapid and direct assay of staphylocoagulase that uses chromogenic substrate Chromozym TH was developed for the routine identification of Staphylococcus aureus. False-positive and false-negative reactions were eliminated by measuring the activity of staphylocoagulase-prothrombin complex directly. The method was compared with three clotting assays. The results show that the new assay is specific, quantitative, and easily automated. If offers clinical laboratories a more reliable assay of staphylocoagulase and is a valuable aid for the rapid identification of S. aureus.

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