Exchange characteristics of the noradrenaline-sensitive calcium store in vascular smooth muscle cells or rabbit ear artery

Abstract

1. The amplitude of the noradrenaline-sensitive Ca stores has been estimated by measuring the amplitude of the transient contraction induced by the agonist in Ca-free solution. or by measuring the amount of 45Ca released under these conditions. 2. The rate of filling of this store after depletion is much faster than the rate of depletion in Ca-free solution, and depends on [Ca]o in the bathing solution. The degree of filling also depends on [Ca]o. 3. At the same [Ca]o the degree of filling is higher in K-depolarized tissues than in control tissues. However at 10 mM-[Ca]o and 5.9 mM-K the amount of Ca taken up by the store is larger than that after loading in 0.2 mM-Ca and 141.4 mM-K, although the tissues remain relaxed during loading at 5.9 mM-K and contracted at 141.4 mM-K. 4. The Ca antagonists D600 and nicardipine selectively block the contraction induced by K depolarization, but do not affect appreciably the noradrenaline-induced contraction. 5. The filling of the store is not significantly reduced by the presence of the Ca antagonists in solutions containing 5.9 mM-K. However these antagonists reduce the degree of filling in K-rich loading solution to a level which is lower than that observed in the control. 6. Mn blocks both the contraction induced by K-rich solution and the tonic component of the noradrenaline-induced contraction and its also inhibits filling of the store. 7. The results suggest that the filling of the store under physiological conditions occurs by a direct pathway between the store and the extracellular medium.