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. 1981 Apr;34(2):61-8.
doi: 10.7883/yoken1952.34.61.

Separation of Clostridium botulinum type A derivative toxin into two fragments

S Kozaki, S Togashi, G Sakaguchi

Separation of Clostridium botulinum type A derivative toxin into two fragments

S Kozaki et al. Jpn J Med Sci Biol. 1981 Apr.

Abstract

Two fragments with molecular weights of 105,000 (fragment I) and 58,000 (fragment II) were separated chromatographically from each other after Clostridium botulinum type A derivative toxin adsorbed onto a QAE-Sephadex column was treated with dithiothreitol and urea. They were antigenic and formed crossing precipitin lines against anti-derivative toxin in agar gel diffusion tests. Upon removal of dithiothreitol and urea by dialysis, the two fragments reassembled to reconstruct the derivative toxin molecule.

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