Experimental (-) emetine myopathy. Ultrastructural and morphometric observations

Abstract

The ultrastructural changes induced by (-) emetine hydrochloride were studied in two skeletal muscles of the rat. (-) Emetine was administered at a dose level of 2 mg/kg for periods ranging from one to four weeks. Changes were noted after the one week stage and were progressive with all muscle components appearing to be affected. Myofibrillar changes included Z line streaming, rod formation, myofilament loss and contraction clumping. Mitochondrial degeneration was detected, but the most striking feature of the induced myopathy was the extensive membrane proliferation. The membranes were considered to be involved in the isolation of sarcoplasmic constitutents in the production of autophagic vacuoles. Morphometry was carried out in order to quantify some of the changes in the fibre components.